Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Rabbit Anti-ATP6V1B1 Polyclonal Antibody

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Katalog-Nummer HPA031847

Size : 100ul

Marke : Atlas Antibodies

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HPA031847

ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1

Anti-ATP6V1B1 Antibody

Polyclonal Antibody against HUMAN ATP6V1B1

0.1 mg/ml

info@clinisciences.com

Immunohistochemistry analysis in human kidney and liver tissues using HPA031847 antibody. Corresponding ATP6V1B1 RNA-seq data are presented for the same tissues.

Product details

Target protein: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Target gene: ATP6V1B1
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000006269 (79%)RAT - ENSRNOG00000013573 (77%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000062834
Concentration: 0.1 mg/ml

Applications

Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Gene name: ATP6V1B1
Alternative gene names: ATP6B1, RTA1B, VATB, Vma2, VPP3,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (4)

Han GH, Yun H, Chung JY, Kim JH, Cho HOncol Rep None2023 Mar 31
Zachar R, Thiel S, Hansen S, Henriksen ML, Skjoedt MO, Skjodt K, Hamzaei Z, Madsen K, Lund L, Hummler E, Svenningsen P, Jensen BLSci Rep , 2022 Sep 24; 12:15955. Epub 2022 Sep 242022 Sep 24