Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Rabbit Anti-PON1 Polyclonal Antibody

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HPA001610

paraoxonase 1

Anti-PON1 Antibody

Polyclonal Antibody against HUMAN PON1

0.1 mg/ml

info@clinisciences.com

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Target protein: paraoxonase 1
Target gene: PON1
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000002588 (78%)RAT - ENSRNOG00000008902 (76%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:200 - 1:500
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Immunohistochemistry (IHC)
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JMProteome Sci , 2011 Nov 17; 9:73. Epub 2011 Nov 172011 Nov 17
Lescuyer P, Pernin A, Hainard A, Bigeire C, Burgess JA, Zimmermann-Ivol C, Sanchez JC, Schifferli JA, Hochstrasser DF, Moll SProteomics Clin Appl , 2008 Jul; 2(7-8):1008-18. Epub 2008 Jul 102008 Jul