rabbit Anti-TRB-3 (Tribbles Homolog 3, Neuronal Cell death-inducible Putative Kinase, SINK, p65-interacting Inhibitor of NF-kappaB) Polyclonal Antibody

Tribbles homolog 3 (TRB-3) is a pseudokinase, shown to act as a potent negative regulator of PPAR?, a master regulator of adipocyte differentiation, and to control adipogenesis. It disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. It interacts also with the NF-kappaB transactivator p65 RELA. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells.

Applications:
Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (direct and indirect): 1:2000-1:5000
Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature
Immunohistochemistry (paraffin sections): 1:500
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.