Recombinant Human M-CSF
Katalog-Nummer GMP-CR97-50ug
Size : 50ug
Marke : Novoprotein
Description - M-CSF, also known as CSF-1, is a four alpha -helical-bundle cytokine, mainly produced by mononuclear macrophages, lymphocytes, endothelial cells, fibroblasts, epithelial cells, etc. M-CSF is the main regulator of macrophage survival, proliferation, and differentiation. M-CSF can promote and enhance the killing of macrophages on tumor cells and microorganisms, regulate the release of cytokines and other inflammatory regulators by macrophages, and stimulate cytophagocytosis. M-CSF also plays an important role in regulating osteoclast proliferation and differentiation, and regulating bone resorption, and is necessary for normal bone development.
- Recombinant Human M-CSF is produced by E.coli expression system, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.
Usage In-vitro cultivation of various types of cell.
Quality Control | Item | Standard |
|---|---|
| Appearance | White powder |
| Reconstitution | Totally dissolve in stated time |
| Visible particle | Conform to ChP |
| pH value | 6.9-7.9 |
| Purity | ≥ 95% |
| Bioactivity | ≥ 6×107 IU/mg |
| Endotoxin | < 10 EU/mg |
| Host protein | ≤ 0.02% |
| Exogenous DNA residue | ≤10 ng/mg |
| Mycoplasma test | Negative |
| Sterility test | Conform to ChP |
| Annotation: ChP refers to the Pharmacopoeia of the People’s Republic of China. | |
Storage Storage at -20±5℃, expiration date labeled on bottle.
Recommendations - （1） Make sure the product properly stored and none expired before using.
- （2） Please use water for injection for reconstitution, to a concentration > 100 μg/ml.
- （3） Sterile condition without pyrogen is required for reconstitution, re-aliquoting, dilution.
- （4） After reconstitution, aliquoted solution vials should be stored at -70°C and minimize freeze-thaw cycles preventing denaturation of protein.
Bioactivity-Cell Based Assay 
Measured in a cell proliferation assay using M-NFS-60 mouse lymphoblast cells.The specific activity of Recombinant human M-CSF is ≥ 6.0 × 107 IU/mg, which is calibrated against human M-CSF WHO International Standard (NIBSC code: 89/512).