CF® Dyes conjugates of Streptavidin are high quality biotin-binding proteins labeled with the superior CF® Dyes or a selection of other labels. These conjugates are typically used as secondary reagents to detect biotinylated probes such as primary antibodies for flow cytometry, western blotting, immunofluorescence staining, and other applications.

• Conjugates with wide choice of CF® Dye colors, plus HRP, R-PE, APC, PerCP, and Alkaline Phosphatase
• Superior CF® Dyes are bright, photostable, and water-soluble
• Ideal for flow cytometry, western blotting, and immunofluorescence staining

Blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.  Learn more about CF® Dyes.

Streptavidin Conjugates

Storage and Handling

Liquid formats:

Streptavidin, R-PE Conjugate and PerCP Conjugate: Store at 4°C, protected from light. Storage at -20°C is not recommended for R-PE or PerCP conjugates. Product is stable for at least 6 months from date of receipt when stored as recommended.

Streptavidin, APC Conjugate: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended.

Streptavidin, Alkaline Phosphatase Conjugate: Store at -20°C. Product is stable for at least 6 months from date of receipt when stored as recommended.

Lyophilized format:

CF® Dye Streptavidin Conjugates: Product is stable for at least 2 years at -20°C with desiccant. Add 0.5 mL of PBS to 1 mg lyophilized CF® Dye Streptavidin Conjugate and mix gently to dissolve.

Streptavidin, HRP Conjugate: add 1 mL pH ~7.4 1X PBS and mix gently to dissolve. Reconstituted HRP conjugate can be stored in aliquots at -20°C for at least 6 months. Do not add azide to HRP conjugates.