Swine Human Ig kappa light chains (free and bound), conjugated with FITC Antibody

Katalog-Nummer orb21578-1ml

Size : 1ml

Marke : Biorbyt


Swine Human Ig kappa light chains (free and bound), conjugated with FITC Antibody

SKU: orb21578

Description

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal swine antiserum to free and bound human Ig kappa light chain. This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the kappa type as well as free light chains or Bence Jones proteins of the kappa type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Research Area

Immunology & Inflammation

Images & Validation

ReactivityHuman

Key Properties

Antibody TypeSecondary Antibody
HostPorcine
ClonalityPolyclonal
ConjugationFITC

Storage & Handling

StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Form/AppearanceFluorochrome coupled purified swine IgG lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Protocol Information