Taurine (Tau) BioAssay™ ELISA Kit (General)

The Taurine (Tau) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of taurine in serum, plasma and other biological fluids.

Sensitivity:
4.39ng/ml

Range:
12.35-1000ng/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of Taurine (Tau). No significant cross-reactivity or interference between Taurine (Tau) and analogues was observed.

Precision:
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Test Principle:
A monoclonal antibody specific to taurine has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled taurine and unlabeled taurine (Standards or samples) with the pre-coated antibody specific to taurine. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of taurine in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of taurine in the sample.

Kit Components:
96-well strip plate, 1x Plate
Standard, 2x vial
Detection Reagent A, 1
Detection Reagent B, 1×120ul
Reagent Diluent, 1×300ul
TMB Substrate, 1×9ml
Wash Buffer (30X), 1×20ml
Standard Diluent, 1×20ml
Assay Diluent A, 1×12ml
Assay Diluent B, 1×12ml
Stop Solution, 1x6ml

Storage and Stability:
Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.