Unit definition: One unit of Vaccinia Capping Enzyme is defined as the amount of enzyme required to incorporate 10 pmol of (α32P) GTP into an 80 nt transcript in 1 hour at 37°C.

– 400 U Vaccinia Capping Enzyme (10 U/µL)
– Reaction Buffer 10x (500 mM Tris-HCl pH=8,0; 10 mM MgCl2; 50 mM KCl; 10 mM DTT)
– GTP (10 mM)
– S-adenosylmethionine (SAM) 40 mM

• Labeling 5´ end of mRNA.
• mRNA vaccine development
• Gene therapy and RNA therapeutics
• In vitro transcription and synthetic biology
• Functional studies on RNA processing

Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

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For optimal results, use fresh RNA transcripts and maintain RNase-free conditions during reactions. Avoid multiple freeze–thaw cycles.

• Shipped in: Gel Pack.
• Storage: -20 ºC (NON Frost-Free Freezer).

This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

To be informed about our Safety Staments for GMP Products, please contact us.

mRNA capping enzyme, Vaccinia virus capping enzyme, RNA 5′ capping enzyme, mRNA cap synthesis enzyme, RNA capping kit enzyme

Also known as:

• Spanish: Enzima de capping de ARN, Enzima de cap 5’, Enzima de capping Vaccinia
• French: Enzyme de coiffage ARN, Enzyme de cap 5’, Enzyme de coiffage Vaccinia
• German: RNA-Capping-Enzym, 5’-Cap-Enzym, Vaccinia-Capping-Enzym
• Italian: Enzima di capping RNA, Enzima cap 5’, Enzima di capping Vaccinia

Q1: Is this enzyme compatible with T7 RNA polymerase?

A: Yes, it is optimized for use with T7 and other commonly used RNA polymerases.

Q2. Can it be used in large-scale mRNA production?

A: Yes, the enzyme is suitable for both small-scale and scalable workflows.