PE-conjugated anti-chicken primary antibodies are affinity-purified antibodies raised against chicken (Gallus gallus) immunoglobulins or specific chicken proteins and covalently labeled with R-phycoerythrin (PE). These one-step fluorescent reagents provide intense fluorescence and are optimized for flow cytometry, multicolor immunofluorescence, and other fluorescence-based assays where high brightness is required. PE conjugates are particularly useful for detecting low-abundance antigens or as part of multicolor panels when maximal signal is needed in a single channel.
Key Features
- High Brightness: R-phycoerythrin exhibits a high extinction coefficient and quantum yield, delivering strong fluorescence signals even when target antigens are expressed at low levels.
- Direct Labeling: Eliminates the need for secondary antibodies, reducing assay time and minimizing cross-species background staining.
- Optimized Fluorophore Conjugation: Controlled dye-to-protein (D/P) ratios and gentle conjugation procedures help preserve both antibody affinity and PE fluorescence performance.
- High Molecular Weight Dye: PE is a large phycobiliprotein fluorophore, and conjugates are specifically formulated to maintain excellent solubility and long-term stability.
- Flexible Formats: Available as monoclonal or polyclonal antibodies in liquid or lyophilized formats, with custom conjugation services offered for specialized applications.
- Quality Control Testing: Each lot is evaluated for dye-to-protein ratio, fluorescence intensity, and functional performance in flow cytometry and immunofluorescence assays.
Common Applications
- Flow Cytometry (FACS): Provides a bright fluorescence channel for single-color and multicolor experiments, making it particularly suitable for low-abundance surface markers and applications requiring a high signal-to-noise ratio.
- Immunofluorescence (IF) and Confocal Microscopy: Delivers strong fluorescence signals for clear visualization of cellular and tissue structures and is compatible with standard PE filter sets.
- Multicolor Panel Design: Commonly used with instruments equipped with 488–561 nm excitation lasers and serves as a highly effective mid-to-bright fluorophore in complex staining panels.
- Fluorescent Immunoassays: Suitable for plate-based assays and bead-based multiplex platforms that require high fluorescence intensity for sensitive detection.
- Cell Sorting: Bright PE fluorescence enhances discrimination of target populations, improving the accuracy of fluorescence-activated cell sorting (FACS), particularly for rare cell subsets.