Acetaldehyde Colorimetric Assay Kit
Katalog-Nummer E-BC-K769-M-48T
Size : 48T
Marke : Elabscience
For research use only.
| Detection Principle | Acetaldehyde is the main aldehyde compound in fermented food such as beer and wine, and has pungent odor. At present, acetaldehyde can be used as a food additive in yogurt, juice, candy, and alcoholic beverages, and it is generally believed that acetaldehyde ingestion from normal drinking in daily life will not cause cancer. However, more and more recent studies have found that acetaldehyde produced in the human bodythrough the pathway of ethanol metabolism will increase the probabilityof human cancer. The detection principle of this kit is: The enzyme catalyzes acetaldehyde and other aldehydes to product an orange-red substance with the chromogenic agent. The maximum absorption of the substance is at 450 nm, and the content of acetaldehyde in the sample can be calculated by measuring the OD value at 450 nm. |
| Sample Type | Serum,Plasma,Wine |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (445-455 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.80 μmol/L |
| Detection Range | 2.80-56.00 μmol/L |
| Precision | inter-assay CV: 2.7-4.0% | intra-assay CV: 2.0-2.1% |
| Sample Volume | 20 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Aged vinegar | 5-10 |
| White spirits | 4-10 |
| Green plum wine | 4-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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