BNUB1365-100 | Adipophilin / Perilipin-2 (Marker of Obesity) (ADFP/1365), 0

Adipophilin / Perilipin-2 Monoclonal Mouse Antibody (ADFP/1365)

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Western Blot of recombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Mouse Monoclonal Antibody (ADFP/1365).

Flow Cytometry of human Adipophilin on PBMC. Green: Isotype Control; Red: Adipophilin Monoclonal Antibody (ADFP/1365).

SDS-PAGE Analysis Purified Adipophilin Mouse Monoclonal Antibody (ADFP/1365). Confirmation of Purity and Integrity of Antibody.

Analysis of Protein Array containing more than 19000 full-length human proteins using Adipophilin Mouse Monoclonal Antibody (ADFP/1365). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.

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Product Description

Perilipin-2, also known as Adipophilin, belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This 48 kDa protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email [email protected] to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 1365, Anti-Adipophilin (ADFP/1365)

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNC74	CF®740	742/767	633-685	775/50	CF®740 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Technology

CF® Dyes

Considerations for Immunofluorescence Staining

Primary & Secondary Antibody Conjugates

Product Attributes

research-areas

Endocrinology, Metabolism

Antibody number

#1365

Size

50 uL, 100 uL, 250 uL, 500 uL

Antibody reactivity (target)

Adipophilin, Perilipin-2

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

ADFP/1365

Isotype

IgG1, kappa

Molecular weight

48 kDa

Synonyms

Perilipin-2; Adipophilin; ADFP; ADRP; Adipose differentiation-related protein; Perilipin-2

Human gene symbol

PLIN2

Entrez gene ID

123 (Human)

SwissProt

Q99541 (Human)

Unigene

3416 (Human)

Immunogen

Recombinant human Adipophilin protein fragment (aa249-376) (exact sequence is proprietary)

Verified antibody applications

Flow (intracellular) (verified), WB (verified)

Antibody target cellular localization

Cytoplasmic

Species reactivity

Human

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Validated in protein array

Monospecific

Antibody research areas