| Short Description : | Rabbit polyclonal anti-LIM domain and actin-binding protein 1 (631-681 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
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| Immunogen: | Synthesized peptide derived from part of the human protein at the amino acid range 631-681 |
| Immunogen Region: | 631-681 aa |
| Specificity: | LIMA1 Polyclonal Antibody detects endogenous levels of protein. |
| Post Translational Modifications | Ubiquitinated by the ECS(RAB40B) complex leading to its degradation. Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility. |
| Function | Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. Acts as a negative regulator of primary cilium formation. Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake. |
| Protein Name | Lim Domain And Actin-Binding Protein 1Epithelial Protein Lost In Neoplasm |
| Database Links | |
| Cellular Localisation | CytoplasmCell JunctionFocal AdhesionCytoskeletonStress FiberCell MembraneCell ProjectionRuffleLamellipodiumExpressed In The Brush Border Membrane Of The Small Intestine And Colocalizes With Npc1l1 And Myo5bColocalizes With Pxn At Focal Adhesions In Mesangial CellsColocalizes With Actin Stress Fibers In Quiescent CellsPdgf Stimulation Induced Disassembly Of Stress Fibers And Formation Of Peripheral And Dorsal RufflesWhere Lima1 Is RelocalizedLocalized At The LamellipodiaJust Behind Lamellipodia Actin Ruffles |
| Alternative Antibody Names | Anti-Lim Domain And Actin-Binding Protein 1 antibodyAnti-Epithelial Protein Lost In Neoplasm antibodyAnti-LIMA1 antibodyAnti-EPLIN antibodyAnti-SREBP3 antibodyAnti-PP624 antibody |
Information sourced from Uniprot.org