Anti-Mouse CD11b - APC
Katalog-Nummer C341-100
Size : 100ug
Marke : Leinco Technologies
AntiMouse CD11b [Clone M1/70] — APC
AntiMouse CD11b [Clone M1/70] — APC
Product No.: C341
Clone M1/70 Target CD11b Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Mac1 αm chain Isotype IgG2b Applications FA , FC , ICC , IHC FF |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Product Concentration 0.2 mg/ml Formulation This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This APC conjugate is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Next Day 28°C Excitation Laser Red Laser (650 nm) RRIDAB_2829659 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this M1/70 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? ICC IHC (Frozen) Additional Reported Applications For Relevant Conjugates ? Depletion FA IHC (Frozen) IHC (Paraffin) IP IF Microscopy For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone M1/70 recognizes the α subunit (CD11b) of the mouse CD11b/CD18 complex. Background LFA1α (CD11a) and CD18 are the Integrin alphaL and beta2 chains respectively that combine to form LFA1, a glycoprotein and a member of the Integrin family. Integrin alphaL/beta2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA1 participates in the immunological synapses between CD8+ T lymphocytes and antigenpresenting cells. The absence of LFA1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyteendothelial cell interaction, cytotoxic Tcell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of Tcells and macrophages. Ligand/Receptor ICAM1 (CD54), ICAM2 (CD102), ICAM4 (CD242), iC3b, fibrinogen PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Innate Immunity . Neuroscience . Neuroscience Cell Markers References & Citations1. Springer, T.A., et al. (1978) Eur. J. Immunol. 8:539 2. Springer, T.A., et al. (1982) Immunol. Rev. 68:171 3. Ding, A., et al. (1987) J Exp Med. 165:733 4. Vignali, D. A. et al. (1990) J. Immunol. 144:4030 5. Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320 |