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Anti-TLR4 Antibody (9Z608)

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Katalog-Nummer NB-64-120883-50uL

Size : 50uL

Marke : Neo Biotech


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Anti-TLR4 Antibody (9Z608)

(Synonyms: toll-like receptor 4, TOLL, TLR-4, TLR4, CD284, ARMD10) Copy Product Info

Synonyms: toll-like receptor 4, TOLL, TLR-4, TLR4, CD284, ARMD10

Catalog No. TMAH-01179 Copy Product Info
Anti-TLR4 Antibody (9Z608) is an antibody targeting TLR4. Anti-TLR4 Antibody (9Z608) can be used in ELISA,FCM.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Description
Anti-TLR4 Antibody (9Z608) is an antibody targeting TLR4. Anti-TLR4 Antibody (9Z608) can be used in ELISA,FCM.
Synonymstoll-like receptor 4, TOLL, TLR-4, TLR4, CD284, ARMD10
Ig Type
hIgG1
Clone
9Z608
Reactivity
Human
Verified Activity
Overlay Peak curve showing Hela cells stained with TMAH-01179 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA
FCM
Recommended Dose
FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Early endosome. Cell projection, ruffle.
Tissue SpecificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Research BackgroundCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific. Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor. Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human TLR4 Protein
Antigen Species
Human
Gene Name
TLR4
Gene ID
7099
Uniprot ID
O00206
Biology Area
Cardiovascular, Immunology, Microbiology
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Keywords

TLR 4CD-284CD 284ARMD-10ARMD 10
Related Tags: Anti-TLR4 Antibody (9Z608) chemical structure