BDNF Protein (OPPA00472)

Datasheets/Manuals	Printable datasheet for OPPA00472

Product Info
Predicted Species Reactivity	Human
Product Format	Lyophilized without additives. Physical appearance: Sterile filtered white powder..
::	Protein Content: BDNF quantitation was carried out by two independent methods. 1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.
Reconstitution and Storage	It is recommended to reconstitute the lyophilized Brain-derived Neurotrophic Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Brain-derived Neurotrophic Factor should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purification	BDNF Human Recombinant is purified by proprietary chromatographic techniques.
Purity	Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity	The ED50, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2 ug/ml.
Source	E.coli

Gene Symbol	BDNF
Gene Full Name	Brain-derived neurotrophic factor
Alias Symbols	ANON2, BULN2
NCBI Gene Id	627
Protein Name	Brain-derived neurotrophic factor
Description of Target	BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. the versatility of bdnf is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Uniprot ID	P23560
Protein Accession #	NP_001137277.1
Protein Size (# AA)	Recombinant
Molecular Weight	27 kDa

Protein interactions

Biological process

Name	# of Products
Anti-apoptosis	239
Axon guidance	286
Axon target recognition	5
Dendrite development	26
Fear response	7
Feeding behavior	36
Glutamate secretion	15
Inner ear development	38
Learning or memory	45
Mechanoreceptor differentiation	5
Negative regulation of neuroblast proliferation	11
Negative regulation of neuron apoptosis	96
Nerve development	9
Nervous system development	384
Neuron recognition	4
Positive regulation of neuron differentiation	80
Regulation of metabolic process	4
Regulation of retinal cell programmed cell death	9
Regulation of synaptic plasticity	28
Response to drug	323
Ureteric bud development	42