ELISA: 1 µg/ml coated antigen can be detected by ≥ 1ng/ml of antibody. The antibody can detect Human B2MG in Sandwich ELISA in combination with capture antibody, anti-B2MG mAb D2E9. Western Blot: 1 µg/ml. Immunofluorescence: 1/50 (20 µg/ml). Fluorescence Flow Cytometry.
Reactivity
Human
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Highly purified recombinant Beta-2-Microglobulin from Human Urine.
Specificity
This monoclonal antibody reacts with natural and recombinant Human Beta-2 MG.
Restore with Double distillated water to a final concentration to 1.0 mg/ml.
Purification
Affinity Chromatography on Protein G.
Conjugation
Unconjugated
Storage
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Human beta 2 microglobulin (B2MG) is a component of major histocompatibility complex 1 (MHC1). It is abundant at the surface of lymphocytes and exists at the surface of all cell types except red cells and platelets. B2MG is normally filtered out of blood through glomeruli, but the process is impaired in patients with renal diseases. Elevated B2MG level is implicated with lymphoma, multiple myeloma, renal diseases, viral infections and auto-immune conditions.
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