Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> CHAPSO [82473-24-3]

CHAPSO [82473-24-3]

Drucken Drucken
Melden Sie sich an

Katalog-Nummer HY-W040201-100mg

Size : 100mg

Marke : MedChemExpress


Beschreibung

CHAPSO is a bile derivative. CHAPSO reduces protein adsorption, improves the cryo-EM imaging quality. CHAPSO can be used as a detergent, or as the membrane protein solubilization and reconstruction reagent in membrane protein structural studies[1][2][3].

In Vitro

CHAPSO prevents bacterial transcription complexes from adsorbing on the air/water interface during cryo-EM grid preparation, thereby avoiding the generation of orientation bias. CHAPSO can be used for Cryo-EM sample preparation and Cryo-EM tomography[2].
CHAPSO forms a monolayer covering the air/water interface at the critical micelle concentration (CMC), changing the surface properties of the interface and preventing particle adsorption[2].
CHAPSO dissolves liposomes and membrane proteins, releasing them from lipid bilayer structures[3].
CHAPSO can be used to prepare phospholipid bilayers with specific orientations for nuclear magnetic resonance experiments[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CHAPSO Related Antibodies

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 100 mg/mL (158.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (158.51 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5851 mL 7.9254 mL 15.8509 mL
5 mM 0.3170 mL 1.5851 mL 3.1702 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Reinheit & Dokumentation
Verweise