Cy5-UTP
Katalog-Nummer B8333-25ul
Size : 25ul(10mM)
Marke : APExBIO Technology
Cy5-UTP (Cyanine 5-UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy5-UTPs. In addition, Cy5-labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis.
Probes generated by these methods are suitable for various application, such as FISH, multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with Cy5-UTP.
Excitation/Emission (nm): 650/670 nm
- 1. Shijing Huang, Yuanyuan Chu, et al. "Fine‐Tuned Regulation of mRNA Translation and Transport by STAU2 Condensate Facilitates Neuronal Development and Plasticity." Adv Sci (Weinh). 2026 May 29:e00044. PMID: 42210778
- 2. Xiaolu Yu, Xiao-Meng Deng, et al. "Nose-to-Brain Delivery of mRNA-Loaded Lipid Nanoparticles Bypasses the Blood–Brain Barrier for Effective Brain Disease Therapy." ACS Nano. 2026 May 19. PMID: 42157518
- 3. Jieli Wei, Yize Zhang, et al. "Multivalent 28S rRNA expansion segments enable reconstitution of multilayered nucleolar architecture." Mol Cell. 2026 Apr 7:S1097-2765(26)00199-1. PMID: 41950918
- 4. Yan Zong, Haiping Zhong, et al. "Safe and Highly Efficient Lipid-Pro-Dexamethasone Nanoparticles for mRNA Delivery and Base Editing." J Am Chem Soc. 2026 Feb 24. PMID: 41733506
- 5. Lijun Hu, David J Peeler, et al. "Polyanion Chemistry Engineers Ternary RNA Nanoparticle Structure/Function from the Inside-Out." ACS Nano. 2026 Feb 10;20(5):4508-4526. PMID: 41592217
- 6. Jingjing Su, Wenguang Huang, et al. "A novel mRNA nanovaccine with dual immunoregulation ameliorates rheumatoid arthritis and associated pneumonia." Asian Journal of Pharmaceutical Sciences Volume 21, Issue 1, February 2026, 101120
- 7. Bookun Kim, Cheol Hui Park, et al. "Size control of lipid nanoparticles via simulation-based design of a microfluidic chip and its effect on mRNA delivery in vitro and in vivo." J Nanobiotechnology. 2025 Nov 23. PMID: 41276828
- 8. Yu Feng, Tongshu Luan, Zhenda Zhang. "Annexin A7 enhances TIA1 axonal trafficking to counteract pathological aggregation in neurons." EMBO J. 2025 Nov 3. PMID: 41188647
- 9. Adarsh Balaji, Simone Hall, et al. "MALAT1 regulates mRNA processing through sequence dependent RNA–RNA and RNA–protein interactions." Nucleic Acids Res. 2025 Aug 11;53(15):gkaf784. PMID: 40808300
- 10. Yanyan Xue, Yi Sun, et al. "Observation of SAM-VI Riboswitch Dynamics Using Single-Molecule FRET." Biomolecules. 2025 Jun 9;15(6):841. PMID: 40563481
- 11. Adarsh Balaji, Aileen C. Button, et al. "The levels of the long non-coding RNA MALAT1 affect cell viability and modulate TDP-43 binding to mRNA in the nucleus." J Biol Chem. 2025 Jan 19;301(3):108207. PMID: 39837396
- 12. Chengzhi Luo, Yunfei Li, et al. "Intracellular trafficking of lipid nanoparticles is hindered by cholesterol." Int J Pharm. 2025 Jan 16:671:125240. PMID: 39826779
- 13. Yiqin Cheng, E Zhao, et al. "Entrapment of lipid nanoparticles in peripheral endosomes but not lysosomes impairs intracellular trafficking and endosomal escape." Int J Pharm. 2025 Jan 25;669:125024. PMID: 39631713
- 14. Yu Feng, Tongshu Luan, et al. "Axon Trafficking Counteracts Aberrant Protein Aggregation in Neurons." bioRxiv - Neuroscience Pub Date : 2025-01-17
- 15. Duxuan Liu, Haoyu Chen, et al. "Design of a Nano-Pesticide Combining Luvangetin and Rnai for Targeted Control, Facilitating Efficient and Eco-Friendly Management of Plant Pathogens." Chemical Engineering Journal Volume 518, 15 August 2025, 164765
- 16. Yi Lin, Mengyao Li, et al. "Tissue-specific mRNA delivery and prime editing with peptide–ionizable lipid nanoparticles." Nat Mater. 2025 Sep 1
- 17. Yeeun Lee, Ji Sun Park, et al. "Lung-targeted delivery of TRAIL and BAK mRNA by optimized lipid nanoparticles for in vivo lung metastasis." Chemical Engineering Journal Volume 522, 15 October 2025, 167379
- 18. Junyong Yoon, Erinn Fagan, et al. "In Situ Tumor-Infiltrating Lymphocyte Therapy by Local Delivery of an mRNA Encoding Membrane-Anchored Anti-CD3 Single-Chain Variable Fragment." ACS Nano. 2024 Nov 26;18(47):32401-32420. PMID: 39527145
- 19. Jie Liu, Guanwen Guan, et al. "SARS-CoV-2 Nucleocapsid Protein Antagonizes GADD34-Mediated Innate Immune Pathway through Atypical Foci." Molecules. 2024 Oct 10;29(20):4792. PMID: 39459161
- 20. Mincheol Jang, Kyunghwan Yeom, et al. "Inhalable mRNA Nanoparticle with Enhanced Nebulization Stability and Pulmonary Microenvironment Infiltration." ACS Nano. 2024 Sep 3;18(35):24204-24218. PMID: 39174871
- 21. Beatriz D Barbieri, David J Peeler, et al. "The role of helper lipids in optimising nanoparticle formulations of self-amplifying RNA." J Control Release. 2024 Oct:374:280-292. PMID: 39142355
- 22. Fei Wang, Guang Cai, et al. "Circular RNA‐based neoantigen vaccine for hepatocellular carcinoma immunotherapy." MedComm (2020). 2024 Jul 29;5(8):e667. PMID: 39081513
- 23. Lingyao Wang, Pilong Li, et al. "Arginine methylation-enabled FUS phase separation with SMN contributes to neuronal granule formation." Cell Rep. 2024 Jul 24;43(8):114537. PMID: 39052476
- 24. Xizhen Lian, Sumanta Chatterjee, et al. "Bone-marrow-homing lipid nanoparticles for genome editing in diseased and malignant haematopoietic stem cells." Nat Nanotechnol. 2024 May 23. PMID: 38783058
- 25. Yang Jiang, Shiqi Sun, et al. "U3 snoRNA inter-regulates with DDX21 in the perichromosomal region to control mitosis." Cell Death Dis. 2024 May 17;15(5):342. PMID: 38760378
- 26. Gege Zeng, Zepeng He, et al. "Cationic Lipid Pairs Enhance Liver-to-Lung Tropism of Lipid Nanoparticles for In Vivo mRNA Delivery." ACS Appl Mater Interfaces. 2024 May 22;16(20):25698-25709. PMID: 38717294
- 27. Colleen A. McHugh, et al. "Dissection of protein and RNA regions required for SPEN binding to XIST A-repeat RNA." RNA. 2024 Feb 16;30(3):240-255. PMID: 38164599
- 28. Subin Kim, Woo Hee Shin, et al. "Direct visualization of replication and R-loop collision using single-molecule imaging." Nucleic Acids Res. 2023 Nov 22:gkad1101. PMID: 37994723
- 29. Binbin Zheng, Liman Niu, et al. "Engineering redirected NF-κB/OIP5/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer." Science Bulletin Volume 68, Issue 24, 30 December 2023, Pages 3207-3224. PMID: 37993335
- 30. Pei Lu, Jie Yang, et al. "A desert lncRNA HIDEN regulates human endoderm differentiation via interacting with IMP1 and stabilizing FZD5 mRNA." Genome Biol. 2023 Apr 24;24(1):92. PMID: 37095549
- 31. Yan Cao, Zongxing He, et al. "Helper-Polymer Based Five-Element Nanoparticles (FNPs) for Lung-Specific mRNA Delivery with Long-Term Stability after Lyophilization." Nano Lett. 2022 Aug 24;22(16):6580-6589 PMID: 35969167
- 32. Shelby L Brown, Dana J Garrison, et al. "Phase separation of a plant virus movement protein and cellular factors support virus-host interactions." PLoS Pathog. 2021 Sep 20;17(9):e1009622. PMID: 34543360
| Physical Appearance | Solution |
| Storage | Store at -70°C or below, protect from light |
| M.Wt | 1178.01(free acid) |
| Formula | C45H58N5O22P3S2(free acid) |
| Synonyms | 5-(3-Aminoallyl)-uridine-5'-triphosphate;labeled with Cy5, Triethylammonium salt, Aminoallyl-UTP-Cy5, Cyanine 5-AA-UTP, Cyanine 5-Aminoallyluridine-5'-Triphosphate, Cyanine 5-UTP |
| Solubility | Soluble in H2O |
| Chemical Name | 2-((1E,3E,5E)-5-(1-(6-(((E)-3-(1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-y |
| Canonical SMILES | O=S(C1=CC2=C(N(CCCCCC(NC/C=C/C3=CN([C@H]4[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O4)C(NC3=O)=O)=O)/C(C2(C)C)=C/C=C/C=C/C(C5(C)C)=[N+](CC)C6=C5C=C(S([O-])(=O)=O)C=C6)C=C1)(O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
