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> Dog Interleukin 6,IL-6 ELISA Kit

Dog Interleukin 6,IL-6 ELISA Kit

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Katalog-Nummer CSB-E11260c-10x96T

Size : 10x96T

Marke : Cusabio

SDS

CSB-E11260c
Size 96T,5×96T,10×96T

Product Details

Target Name
interleukin 6 (interferon, beta 2)
Associated names
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 pg/mL-100 pg/mL
Sensitivity
0.39 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-88  
1:2 Average % 95  
Range % 89-105  
1:4 Average % 100  
Range % 95-106  
1:8 Average % 93  
Range % 89-97  
Recovery
The recovery of dog IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-96  
EDTA plasma (n=4) 93 85-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.954 2.989 2.972 2.855  
50 2.388 2.386 2.387 2.270  
25 1.758 1.796 1.777 1.660  
12.5 1.035 1.106 1.071 0.954  
6.25 0.502 0.513 0.508 0.391  
3.12 0.271 0.268 0.270 0.153  
1.56 0.184 0.196 0.190 0.073  
0 0.112 0.121 0.117 　  
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
Interleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in immune responses, inflammation, and hematopoiesis. Originally identified as a B-cell stimulatory factor, IL-6 regulates acute-phase responses, promotes T-cell activation, and influences the differentiation of various immune cell populations. Multiple cell types produce this cytokine, including macrophages, T-cells, endothelial cells, and fibroblasts in response to inflammatory stimuli. IL-6 signaling occurs through binding to its receptor complex, triggering downstream pathways that affect gene expression and...

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Target Background

Function
(From Uniprot)
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the...
Gene References into Functions
  1. An increase in ventricular dimension is accompanied by increases in AQP4 and IL-6 concentrations in the CSF of dogs with idiopathic communicating hydrocephalus. All were greatly reduced or normalised after shunt treatment. Therefore AQP4 and IL-6 in CSF can be indicators of ventriculomegaly, of cellular damage, and of improvement after the treatment of hydrocephalus.
  2. The results of this study suggest that IL-6 is a useful prognostic marker for canine critical care patients with acute internal disease.
  3. This diminished TNF-alpha, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-kappaB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 microM and 50 microM propofol.
  4. Release of interleukin-6 and substance P may initiate proinflammatory effects leading to development of persistent pain in dogs with syringomyelia.
  5. The etiology of blindness in dogs with pituitary dependent hypercortisolism, including the role of adiponectin, interleukin 6, insulin, and nitric oxide, is reported.
  6. There was no association between IL6 variants and periodontal disease in dogs.
  7. These findings suggest that IL-6 secretion during acute urinary tract infection may serve a dual biological role by initiating the inflammatory response while also repairing urothelial defenses.
  8. Tumor-infiltrating lymphocytes isolated from regressing canine transmissible venereal tumor secrete high concentrations of IL-6 and antagonize the anti-lymphokine-activated killer activity of tumor cell transforming growth factor-beta 1.
  9. Plasma IL-6 concentration is predictive of outcome in canine systemic inflammatory response syndrome and sepsis and may be a valuable laboratory parameter for assessing critically ill dogs.
  10. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10.

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Subcellular Location
Secreted.
Protein Families
IL-6 superfamily
Database Links

KEGG:

STRING:

UniGene: