GluA1/GluR1 Glutamate Receptor Antibody
Katalog-Nummer OASE00288
Size : 100ug
Marke : Aviva Systems Biology
GluA1/GluR1 Glutamate Receptor Antibody (OASE00288)
| Datasheets/Manuals | Printable datasheet for GluA1/GluR1 Glutamate Receptor Antibody (OASE00288) |
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| COA Datasheet | 1 µg/ml of SMC-440 was sufficient for detection of GluA1/GluR1 in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Clonality | Monoclonal |
| Clone | S355-1 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | Unconjugated |
| Application | ICC,IF,IHC,WB |
| Additional Information | Background Info: Detects ~100kDa. Does not cross-react with GluR2. |
| :: | Scientific Background: Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration (3). |
| :: | Certificate of Analysis: 1ug/mL was sufficient for detection of GluA1/GluR1 in 20ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Reconstitution and Storage | -20°C |
| Immunogen | Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1 |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Specificity | Detects ~100kDa. Does not cross-react with GluR2. |
| Dilution | WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Storage Buffer | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
| Description | Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration (3). |
| Reference | 1. Potier M.C., Spillantini M.G., Carter N.P. (1992) DNA Seq. 2(4): 211-218. 2. Puckett, C., et al. (1991) Proc. Nat. Acad. Sci. USA 88(17): 7557-7561. 3. Gregor, P., et al. (1993) Proc. Nat. Acad. Sci. USA 90: 3053-3057. |
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| Gene Symbol | Gria1 |
| Gene Full Name | glutamate ionotropic receptor AMPA type subunit 1 |
| Alias Symbols | AMPA-selective glutamate receptor 1, GluA1, gluR-1, gluR-A, gluR-K1, GluR1, glutamate receptor 1, glutamate receptor A, Glutamate receptor ionotropic, AMPA 1, glutamate receptor subunit GluR1, glutamate receptor, ionotropic, AMPA 1, glutamate receptor, ionotropic, AMPA1 (alpha 1). |
| NCBI Gene Id | 50592 |
| Protein Name | Glutamate receptor 1|glutamate receptor 1 precursor [Rattus norvegicus] |
| Description of Target | Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. |
| Uniprot ID | P19490 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_113796 |
