Human Limbic system-associated membrane protein (LSAMP) ELISA Kit
Katalog-Nummer AE34942HU-48
Size : 48Tests
Marke : Abebio
Reagent Preparation
Results demonstration
Assay Procedure Summary
Certificate
Product Details
| Species Reactivity | Human (Homo sapiens) |
| UniProt | Q13449 |
| Abbreviation | LSAMP |
| Alternative Names | FLJ34254; FLJ35396; FLJ37216; FLJ54658; IGLON3; LAMP; IgLON family member 3 |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1 - 2 0 0 μ L |
| Detection Wavelengt | 450 nm |
Test principle
This assay employs a two-site sandwich ELISA to quantitate LSAMP in samples. An antibody specific for LSAMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LSAMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LSAMP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LSAMP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview
Components
Reagents | Quantity | Reagents | Quantity |
Assay plate (96 Wells) | .label-data{font-weight:bold;min-width:100px;}.label-data,.data{display:inline-block;min-width:100px;}.description table{word-break:break-all;}.description table td{min-width:200px;}
|