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> LdcA, Recombinant, E. coli, aa1-304, His-Tag (Murein Tetrapeptide Carboxypeptidase)

LdcA, Recombinant, E. coli, aa1-304, His-Tag (Murein Tetrapeptide Carboxypeptidase)

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Katalog-Nummer 374000-100ug

Size : 100ug

Marke : US Biological


374000 LdcA, Recombinant, E. coli, aa1-304, His-Tag (Murein Tetrapeptide Carboxypeptidase)

Swiss Prot
P76008
Grade
Purified
Shipping Temp
Blue Ice
Storage Temp
-20°C

Releases the terminal D-alanine residue from the Cytoplasmic domain tetrapeptide recycling product L-Ala-gamma-D-Glu-meso-Dap-D-Ala. To a lesser extent, can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc-tetrapeptide, GlcNAc-MurNAc-tetrapeptide, and GlcNAc-anhMurNAc-tetrapeptide. Does not act on murein sacculi or cross-linked muropeptides. The tripeptides produced by the LcdA reaction can then be reused as peptidoglycan building blocks; LcdA is thereby involved in murein recycling. Is also essential for viability during stationary phase.

Source:
Recombinant full length protein corresponding to aa1-304 from E. coli LdcA, fused to His-Tag at N-terminal, expressed in E. coli.

Molecular Weight:
~37.6kD

Amino Acid Sequence:
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQGEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILNNTREGTQLTISGHPVLKM

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Source: Recombinant, E. coli|Purity: ~90% (SDS-PAGE)|Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Purity
~90% (SDS-PAGE)
References
1. A defect in cell wall recycling triggers autolysis during the stationary growth phase of Escherichia coli.Templin M.F., Ursinus A., Hoeltje J.-V.EMBO J. 18:4108-4117(1999).