Orb779885-48Tests | Mouse V-Ets Erythroblastosis Virus E26 Oncogene

Mouse V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) ELISA Kit

SKU: orb779885

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Description

The Mouse V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) ELISA Kit uses a sandwich enzyme immunoassay to quantify V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) in mouse serum, plasma and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.052 ng/mL.

Research Area

Infectious Disease & Virology; Cancer Biology; Immunology & Inflammation

Images & Validation

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Tested Applications	ELISA
Application Notes	standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ETS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ETS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ETS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ETS1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ETS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ETS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ETS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ETS1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.052 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EWSR2 ELISA Kit, p54 ELISA Kit, Protein C-ets-1 ELISA Kit

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Mouse ETS1 ELISA Kit (Ready to Use) [orb554253]
Reactivity:
Mouse
Dynamic Range:
0.156-10 ng/mL
Sensitivity:
0.042 ng/mL
Sizes Available: 48 T, 96 T
Mouse ETS1 ELISA Kit [orb439376]
Reactivity:
Mouse
Dynamic Range:
0.156-10 ng/mL
Sensitivity:
0.042 ng/mL
Sizes Available: 48 T, 96 T
Mouse V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) ELISA Kit [orb1666546]
Reactivity:
Mouse
Sizes Available: 48 T, 96 T