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> PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx™

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx™

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Katalog-Nummer 31050-X

Size : 1kit

Marke : Biotium

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx™

PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)

A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.

Viability dye
Size
Catalog #
Qty
200 assays
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Product Description

This kit is designed to perform viability PCR on E. coli. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the uidA gene.

Features

  • Selectively detect viable E. coli using qPCR
  • Contains primers to amplify the uidA gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
  • Sufficient reagents for 200 PCR reactions

Kit Components

  • PMA dye or PMAxx™ dye
  • PMA Enhancer for Gram Negative Bacteria
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • uidA primer mix, 5 uM

About this kit

Escherichia coli is a species of bacteria that is commonly used in laboratories. Some strains of E. coli are food-borne pathogens and can cause digestive illness. For specific detection of the pathogenic E. coli strain O157:H7, please see kit number 31037. PCR to amplify the gene uidA has been published and shown to be highly specific for E. coli. The primers provided in the kit have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
MRSAmecA31036N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don't see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.
Technology
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Viability PCR
The Full Spectrum Blog
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12 years later: Viability PCR using PMA and PMAxx™ dyes
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Switching from agar plates to v-PCR for bacteria viability testing – What you need to know

Product Attributes

Size
200 assays
Viability dye
PMA, PMAxx™
Storage Conditions
Store at -10 to -35 °C
Assay type/options
Live/dead discrimination, Viability PCR
Detection method/readout
PCR/qPCR, HRM®

Documents, Protocols, SDS and COA

References

Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.

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