Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Rabbit Anti-DNAI1 Polyclonal Antibody

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Katalog-Nummer HPA021649

Size : 100ul

Marke : Atlas Antibodies

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HPA021649

dynein, axonemal, intermediate chain 1

Anti-DNAI1 Antibody

Polyclonal Antibody against HUMAN DNAI1

0.2 mg/ml

info@clinisciences.com

Immunohistochemistry analysis in human testis and prostate tissues using HPA021649 antibody. Corresponding DNAI1 RNA-seq data are presented for the same tissues.

Product details

Target protein: dynein, axonemal, intermediate chain 1
Target gene: DNAI1
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000061322 (86%)RAT - ENSRNOG00000013734 (80%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000047095
Concentration: 0.2 mg/ml

Applications

Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:500 - 1:1000
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody
Western Blot (WB)Recombinant expression validation in WB using target protein overexpression.

Target information

Protein name: dynein, axonemal, intermediate chain 1
Gene name: DNAI1
Alternative gene names: CILD1, DIC1, PCD,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (15)

Tsai M, Rayner RE, Chafin L, Farkas D, Adair J, Mishan C, Mallampalli RK, Kim SH, Cormet-Boyaka E, Londino JDAm J Physiol Lung Cell Mol Physiol , 2023 Feb 28; 324(5):L666-L676. Epub 2023 Feb 282023 Feb 28
Zhou S, Liu Y, Yang Y, Huang H, Qiu QJ Inflamm Res , 2023 Jan 30; 16:373-380. Epub 2023 Jan 302023 Jan 30