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> Rabbit Mouse IgE (Fc specific), conjugated with Biotin Antibody

Rabbit Mouse IgE (Fc specific), conjugated with Biotin Antibody

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Katalog-Nummer orb21623-1ml

Size : 1ml

Marke : Biorbyt


Rabbit Mouse IgE (Fc specific), conjugated with Biotin Antibody

SKU: orb21623
This product can be custom made

Description

Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to mouse IgE, Fc specific. In immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:3,000.

Research Area

Immunology & Inflammation

Images & Validation

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Rabbit Mouse IgE (Fc specific), conjugated with Biotin Antibody
ReactivityMouse

Key Properties

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Antibody TypeSecondary Antibody
HostRabbit
ClonalityPolyclonal
ConjugationBiotin

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Form/AppearanceBiotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information