Orb781893-48Tests | Rat Spleen Focus Forming Virus Proviral Integration

Rat Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit

SKU: orb781893

Featured

Description

The Rat Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit uses a sandwich enzyme immunoassay to quantify Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) in rat tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.114 ng/mL.

Research Area

Infectious Disease & Virology; Cancer Biology; Stem Cell & Developmental Biology

Images & Validation

−

Tested Applications	ELISA
Application Notes	standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPI1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPI1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Rat
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.114 ng/mL
Concentration	20 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

OF ELISA Kit, PU.1 ELISA Kit, SFPI1 ELISA Kit, SPI-1 ELISA Kit, SPI-A ELISA Kit, SPI-1 Proto-Oncogene ELISA Kit, Hematopoietic Transcription Factor PU.1 ELISA Kit, 31 kDa Transforming Protein ELISA Kit

−

Rat SPI1 ELISA Kit (Ready to Use) [orb555542]
Reactivity:
Rat
Dynamic Range:
0.312-20 ng/mL
Sensitivity:
0.116 ng/mL
Sizes Available: 96 T, 48 T
Rat SPI1 ELISA Kit [orb442335]
Reactivity:
Rat
Dynamic Range:
0.312-20 ng/mL
Sensitivity:
0.116 ng/mL
Sizes Available: 48 T, 96 T
Rat Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit [orb1663450]
Reactivity:
Rat
Sizes Available: 96 T, 48 T