S100A10 ELISA Kit (Human)
Katalog-Nummer OKEH01947
Size : 96T
Marke : Aviva Systems Biology
S100A10 ELISA Kit (Human) : 96 Wells (OKEH01947)
| Datasheets/Manuals | Printable datasheet for S100A10 ELISA Kit (Human) : 96 Wells (OKEH01947) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology S100A10 ELISA Kit (Human) (OKEH01947) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100A10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100A10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100A10 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/ml | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.8% (n = 20) Mean Inter-assay CV%: < 6.7% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.174 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Apelin receptor | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | APLNR |
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| Gene Full Name | apelin receptor |
| Alias Symbols | AGTRL1, angiotensin II receptor-like 1, angiotensin receptor-like 1, apelin receptor, APJ, APJ (apelin) receptor, APJ receptor, APJR, G protein-coupled receptor APJ, G-protein coupled receptor APJ, G-protein coupled receptor HG11, HG11, HG11 orphan receptor. |
| NCBI Gene Id | 187 |
| Protein Name | Apelin receptor |
| Description of Target | Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity (PubMed:11090199, PubMed:25639753, PubMed:28137936). Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone (By similarity). May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis (By similarity). Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development (By similarity). Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure (PubMed:25639753, PubMed:28137936). |
| Uniprot ID | P35414 |
| Protein Accession # | NP_005152.1 |
| Nucleotide Accession # | NM_005161.4 |
| Protein Size (# AA) | 380 |
| Molecular Weight | 43 kDa |
