SCRIPT RT-qPCR SybrMaster UNG is designed for quantitative real-time analyses of RNA templates using the fluorescent DNA stain SYBR Green. The ready-to-use mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The 2x conc. mix contains all reagents required for RT-qPCR (except template and primers) allow fast and easy preparation with a minimum of pipetting steps. The premium quality enzymes and the optimized reaction buffer ensure superior real time PCR results. The mix contains UNG (Uracil-N-Glycosylase) and dUTP instead of
