Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose-1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent protein.

Agaricus bisporus Lectin (ABA/ABL) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.

Applications: Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Glycobiology.

Recommended Usage: 1XPBS pH 7.4 can be used to dilute the stock. Recommended dilution range from 0.5-10 ug/ml. 5-10 µg/ml will agglutinate type A or B cells. 2 µg/ml will agglutinate type O cells. Less than 0.5 µg/ml will agglutinate neuraminidase treated erythrocytes.

bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunofluorescence, Glycobiology

References

Batterbury, M., Tebbs, C. A., Rhodes, J. M., & Grierson, I. (2002). Agaricus bisporus (edible mushroom lectin) inhibits ocular fibroblast proliferation and collagen lattice contraction. Experimental eye research, 74(3), “370. https://doi.org/10.1006/exer.2001.1133

Kent, D., Sheridan, C. M., Tomkinson, H. A., White, S. J., Hiscott, P., Yu, L., & Grierson, I. (2003). Edible mushroom (Agaricus bisporus) lectin inhibits human retinal pigment epithelial cell proliferation in vitro. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 11(4), “291. https://doi.org/10.1046/j.1524-475x.2003.11408.x