AKT (Thr342) Antibody
Cat# bs-70012R
Size : 100uL
Brand : Bioss
Applications
Reactivity
| Overview | |
| Catalog # | bs-70012r |
| Product Name | AKT (Thr342) Antibody |
| Applications | WB |
| Specificity | Specific for endogenous levels of the ~68 kDa AKT protein phosphorylated at Thr342. Immunolabeling is completely eliminated with _-phosphatase treatment. It has been reported that this antibody may also recognize some level of phosphorylated S6K as there is 67% homology with the sequence used as antigen. |
| Reactivity | Drosophila |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 of Drosophila AKT, conjugated to keyhole limpet hemocyanin (KLH) |
| Modification Site | Thr342 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target | |
| Gene ID | 41957 |
| Swiss Prot | Q8INB9 |
| Synonyms | AKT1 antibody, AKT1_HUMAN antibody, AKT2 antibody, AKT3 antibody, PKB alpha antibody, PKB antibody, PKB beta antibody, PKBalpha antibody, PRKBA antibody, PRKBB antibody, PRKBG antibody, Protein kinase B antibody, Protein kinase B beta antibody, Protein kinase B gamma antibody, Proto-oncogene c-Akt antibody, RAC alpha antibody, RAC antibody, RAC gamma antibody, RAC PK alpha antibody, RAC PK beta antibody, RAC PK gamma antibody, RAC-alpha serine/threonine-protein kinase antibody, RAC-PK-alpha antibody, STK2 antibody |
| Background | The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (_, _, and _) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Akt_ occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004). |
| Application Dilution | |
| WB | 1:300-5000 |