Jacalin lectin (AIA) is purified by affinity chromatography and features a tetrameric two-chain structure composed of four identical protomers, with an isoelectric point of pH 7.8. This lectin specifically targets blood group types O (+SA) and T and is recognized as a galactose-specific lectin, eluting with galactose or melibiose. A post-translational proteolytic modification at the N terminus of the α-chain creates a novel carbohydrate-binding site, which underlies its specificity for the T-antigen disaccharide Galβ1,3GalNAc. Inhibitors of jacalin include D-galactose, β-Met-Gal, and 2-deoxy-α-D-galactose.

Cy5 is conjugated to AIA to visualize the binding pattern of this lectin in cellular imaging and flow cytometry; its excitation wavelength is sufficiently high to prevent overlap with most other fluorochromes, facilitating dual-labeling experiments while minimizing background autofluorescence in biological specimens.

Recommended Usage: Recommended dilutions 0.5 – 10 µg / ml in 1XPBS. Less than 1 µg / ml will agglutinate rabbit erythrocytes.

Application

Blotting, Glycobiology, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Mitogenic Stimulation, Cell selection.

References

1. Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
2. Mohamad SFS et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.: Mater. Sci. Eng. 716 012019.
3. Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
4. Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
5. VIJAYAKUMAR T and FORRESTER JA. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.