Reagents and instruments for immunology, cell biology and molecular biology.

Bobcat339 [2280037-51-4]

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Cat# T5198-50mg

Size : 50mg

Brand : TargetMol

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Bobcat339

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Bobcat339 is a novel cytosine-based TET enzyme inhibitor (IC₅₀s: 33/73 uM for TET1/2), but not DNMT3a, does not inhibit the DNA methyltransferase DNMT3a.

Cas No. 2280037-51-4

With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

Batch Information

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Purity:99.67%

Appearance:Solid

Color:White to Cyan

Resource Download

COA HNMR LCMS

Product Information

Bioactivity

Description	Bobcat339 is a novel cytosine-based TET enzyme inhibitor (IC₅₀s: 33/73 uM for TET1/2), but not DNMT3a, does not inhibit the DNA methyltransferase DNMT3a.
Targets & IC50	TET1:33 μM (cell free), TET2:73 μM (cell free)
In vitro	HT-22 cells were treated with 10 μM Bobcat339 in 1% DMSO for 24 hrs and lysed. Cells treated with Bobcat339 had significantly reduced global 5hmC levels as compared to vehicle control.

Chemical Properties

Molecular Weight	297.74
Formula	C₁₆H₁₂ClN₃O
Cas No.	2280037-51-4
Smiles	Nc1nc(=O)n(cc1Cl)-c1cccc(c1)-c1ccccc1
Relative Density.	1.33 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 11.59 mg/mL (38.93 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (6.72 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.3586 mL	16.7932 mL	33.5864 mL	167.9318 mL
5 mM	0.6717 mL	3.3586 mL	6.7173 mL	33.5864 mL
10 mM	0.3359 mL	1.6793 mL	3.3586 mL	16.7932 mL
20 mM	0.1679 mL	0.8397 mL	1.6793 mL	8.3966 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Product Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

TET2TET1epigeneticsDNMTsDNAMethyltransferaseDNA MTasesDNA methyltransferase (DNMT)DNA MethyltransferaseCytosine-based,TETBobcat-339Bobcat339Bobcat 339

Related Tags: Bobcat339 chemical structure | Bobcat339 in vitro | Bobcat339 formula | Bobcat339 molecular weight