RayBio® COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgM antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.
COVID-19 Human IgM ELISA Kit
COVID-19 Human IgM ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Compatible Sample Types | Serum |
| Gene Symbols | IGM |
| Quantitative/Semi-Quantitative | Semi-Quantitative, Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, COVID-19 |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -80°C |
| Antigen | N-Protein, N and S (S1RBD), S-Protein (S1RBD) |
| Sample type | Serum, Plasma, Dried blood |
Application Notes
Kit Components
- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Negative Control
- HRP-anti positive control IgM Antibody
- HRP-anti Human IgM Antibody
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 μl positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
- Add 100 μl prepared HRP-anti positive control IgM Antibody and/or 100 μl of prepared HRP-anti Human IgM Antibody to each well. Incubate 1 hour at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
documents
citations
Inflammatory Leptomeningeal Cytokines Mediate COVID-19 Neurologic Symptoms in Cancer Patients
- Species:
- Human
- Sample Type:
- Plasma, CSF (Inflammation in the brain during covid)
- Year:
- 2021
- Journal Name:
- Cancer Cell
- PubMed ID:
- 33508216
Vertical Transmission of SARS-CoV-2–Specific Antibodies and Cytokine Profiles in Pregnancy
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Journal of Infectious Diseases
Refocus on Immunogenic Characteristics of Convalescent COVID-19 Challenged by Prototype SARS-CoV-2
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Vaccines
Clinico-Epidemiological Laboratory Findings of COVID- 19 Positive Patients in a Hospital in Saudi Arabia
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Infection and drug resistance
Serum N-glycomic profiling may provide potential signatures for surveillance of COVID-19
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Glycobiology
