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Mouse TNF-alpha ELISA Kit

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Cat# ELM-TNFa-5

Size : 5PlateKit

Brand : RayBiotech


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Mouse TNF-alpha ELISA Kit Standard CurveMouse TNF-alpha ELISA KitMouse TNF-alpha ELISA KitRayBiotech’s ELM-TNFα, ELM-MCP1, ELM-IL6, and ELM-IL1β ELISA kits were used to measure serum TNF-α, MCP-1, IL-6, and IL-1β in high-fat diet–fed adipocyte-specific TRPM7 knockout mice. Figure adapted from Zhong et al., Nature Communications, 2023.Figure adapted from Sun et al., Journal of Translational Medicine, 2025.Figure adapted from Wang et al., Journal of Ethnopharmacology, 2025.Figure adapted from Rodríguez-Ramírez et al., Frontiers in Immunology, 2024.ELM-TNFα and ELM-IL6 kits were used to measure TNF-α and IL-6 levels in UVB-irradiated mouse skin. Figure adapted from <a href="https://www.sciencedirect.com/science/article/pii/S2213231723000678" target="_blank">Kim et al., Redox Biology, 2023</a>.Mouse TNF-alpha ELISA KitMouse TNF-alpha ELISA KitMouse TNF-alpha ELISA Kit5 Plate Kit Packaging (one box)

Mouse TNF-alpha ELISA Kit Standard Curve

Mouse TNF-alpha ELISA Kit

RayBio® Mouse TNF alpha ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesMouse
Sensitivity
60 pg/ml (sensitivity of lysate is 94 pg/ml)

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
60 pg/ml - 6000 pg/ml
(detection range of lysate is 94 pg/ml - 6000 pg/ml)
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P06804
Gene Id
21926
Gene Symbols
TNF, TNFSF2
Protein Name / Synonyms
Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCardiovascular Disease, Cancer, Inflammation
Shipping TypeBlue ice
Storage≤-20°C

Target Background

Tumor necrosis factor alpha (TNF alpha), also known as lymphotoxin and cachectin, is a pleiotropic cytokine. TNF alpha is a smaller cytokine with a molecular weight of 26 kDa (transmembrane) and 17 kDa (soluble) that binds to the same receptors producing a vast array of effects similar to those of TNF-beta. Both TNF-alpha and TNF-beta share 30% amino acid homology and have similar biological activities. TNF-alpha is produced primarily by monocytes, neutrophils and macrophages. It is found in synovial cells and macrophages in the tissues. TNF-alpha and TNF-beta can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 (55 kDa) and 2 (75 kDa) to be killed, with the p55 receptor mediating the cytotoxic response.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

RayBiotech’s ELM-TNFα, ELM-MCP1, ELM-IL6, and ELM-IL1β ELISA kits were used to measure serum TNF-α, MCP-1, IL-6, and IL-1β in high-fat diet–fed adipocyte-specific TRPM7 knockout mice. Figure adapted from Zhong et al., Nature Communications, 2023.

RayBiotech’s ELM-TNFα, ELM-IFNγ, ELM-IL1β, ELM-IL17, ELM-IL6, and ELM-IL10 ELISA kits were used to measure TNF-α, IFN-γ, IL-1β, IL-17A, IL-6, and IL-10 in colon tissue homogenates from DSS-induced colitis mice with or without white kidney bean supplementation. Figure adapted from Sun et al., Journal of Translational Medicine, 2025.

RayBiotech’s ELM-TNFα, ELM-IL4, ELM-IL6, ELM-IL10, ELM-IL1β, and ELM-IL17 ELISA kits were used to measure serum TNF-α, IL-4, IL-6, IL-10, IL-1β, and IL-17a in DSS-induced colitis mice treated with Shaoyao decoction. Figure adapted from Wang et al., Journal of Ethnopharmacology, 2025.

RayBiotech’s ELM-TNFa-CL-1, ELM-IFN-γ-CL-1, and ELM-IL1β-CL-1 ELISA kits were used to measure TNF-α, IFN-γ, and IL-1β levels in matched retinal and plasma samples from male and female mice following systemic LPS administration. Figure adapted from Rodríguez-Ramírez et al., Frontiers in Immunology, 2024.

RayBiotech’s ELM-TNFα and ELM-IL6 ELISA kits were used to measure TNF-α and IL-6 levels in UVB-irradiated mouse skin. Figure adapted from Kim et al., Redox Biology, 2023.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Mouse TNF-alpha ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified TNF alpha (TNFA), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

citations

  • Acarbose modulates microglial Pkm2 acetylation to reshape immunometabolism and preserve retinal neurons after ischemia-reperfusion

    Species:
    Mouse
    Sample Type:
    Other / Unspecified (retinas)
    Year:
    2026
    Journal Name:
    Journal of Neuroinflammation
    PubMed ID:
    42069589

  • Icariin alleviates corneal neovascularization by regulating inflammation through inhibition of macrophage polarization and the NF-κB pathway

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (corneal tissue homogenate supernatant, in vitro samples)
    Year:
    2026
    Journal Name:
    Journal of Translational Medicine
    PubMed ID:
    41652511

  • Oral Delivery of Mesenchymal Stem Cell-Derived Extracellular Vesicles To Treat Intestinal Inflammation

    Year:
    2026
    Journal Name:
    ACS Applied Materials & Interfaces
    PubMed ID:
    42376871

  • Cataract Aggravates Alzheimer-Like Pathologies and Cognitive Deficits in an APP/PS1 Mouse Model

    Species:
    Mouse
    Sample Type:
    Tissue Lysate / Homogenate (brain homogenates)
    Year:
    2026
    Journal Name:
    Neuroscience Bulletin
    PubMed ID:
    40580389

  • WKB ameliorates DSS-induced colitis through inhibiting enteric glial cells activation and altering the intestinal microbiota

    Year:
    2025
    Journal Name:
    Journal of Translational Medicine
    PubMed ID:
    39838431

  • Reversing the "cold" tumor microenvironment: the role of neoantigen vaccines in prostate cancer

    Species:
    Mouse
    Sample Type:
    Tissue Lysate / Homogenate (tumor tissue lysates)
    Year:
    2025
    Journal Name:
    Journal of Translational Medicine
    PubMed ID:
    40713775

  • Anti-Inflammatory Effects of Marine-Derived Resorcylic Acid Lactone Derivatives in Ulcerative Colitis via the MAPK/ERK Pathway

    Species:
    Mouse
    Sample Type:
    Serum (colitis mice)
    Year:
    2026
    Journal Name:
    Marine Drugs
    PubMed ID:
    41745472

  • Novel ethosomal gel formulation for enhanced transdermal delivery of curcumin and cyclosporine: a preclinical approach to rheumatoid arthritis management

    Species:
    Mouse
    Sample Type:
    Other / Unspecified (LPS-stimulated RAW 264.7 cells)
    Year:
    2025
    Journal Name:
    Drug Delivery
    PubMed ID:
    40458935

  • MHC-I upregulation by macbecin II in the solid tumors potentiates the effect of active immunotherapy

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (co-culture medium of OT-1 cells, B16-Ova cells)
    Year:
    2025
    Journal Name:
    EMBO Molecular Medicine
    PubMed ID:
    40087501

  • Aging and MPTP Sensitivity Depend on Molecular and Ultrastructural Signatures of Astroglia and Microglia in Mice Substantia Nigra

    Species:
    Mouse
    Sample Type:
    Tissue Lysate / Homogenate (protein lysates from ventral midbrains)
    Year:
    2025
    Journal Name:
    Cellular and Molecular Neurobiology
    PubMed ID:
    39833644

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