Extraction and purification of plasmidic DNA - Maxiprep
Plasmid preparation is the extraction and purification of plasmid DNA from bacteria for example. Whatever the method used, there are always 3 main steps: cultivation of host organisms (such as bacteria), lysis to recover the plasmids and purification of the plasmid DNA.
There are many kits on the market for making plasmid preparations and these kits are classified according to the volume of culture and the corresponding quantity of plasmids. A distinction is made between Minipreps (for mini-preparation), Midipreps, Maxipreps, Megapreps and Gigapreps.
The mini-prep or Miniprep corresponds to the smallest quantity of plasmid DNA, between 5 and 50 µg for a culture volume of a few millilitres.
The midiprep corresponds to a medium quantity of plasmid DNA, between 100 and 350 µg for a culture volume of 15-25 ml.
The Maxiprep corresponds to a larger quantity of plasmid DNA, between 500 and 850 µg for a culture volume of 100-200 ml.
Frequently Asked Questions (FAQ)
How can I quickly find a maxiprep product?
To find a maxiprep product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does CliniSciences stock several product codes in the maxiprep category?
Yes, CliniSciences distributes several product references in the maxiprep category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for maxiprep products?
Yes, you can request a quote for maxiprep products from the product page or by contacting CliniSciences (info@clinisciences.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a maxiprep product is available to order?
The availability of a maxiprep product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact CliniSciences with the product code or name of the product you are looking for.
What should I do if I cannot find the maxiprep product I am looking for?
If the maxiprep product you are looking for does not appear in the catalogue, please contact CliniSciences with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all maxiprep products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.
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