Cd80 Rat Monoclonal Antibody [Clone ID: 1G10]

Specifications

Product Data
Clone Name	1G10
Applications	FC
Recommended Dilution	Flow Cytometry: < / = 0.2 µg/10e6 cells. (Ref.2,7)
Reactivities	Mouse
Host	Rat
Isotype	IgG2a
Clonality	Monoclonal
Specificity	This antibody recognises CD80/B7-1 (Mr. 45-47 kDa)
Formulation	PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent. Label: APC State: Liquid purified Ig fraction. Label: Allophycocyanin
Concentration	lot specific
Conjugation	APC
Storage	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	CD80 antigen
Database Link	Entrez Gene 12519 Mouse UniProt ID Q00609
Background	CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. (Ref.1-4) The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. (Ref.5,6). This Monoclonal antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. (Ref.2,7).
Synonyms	CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1
Reference Data