Cd3e Rat Monoclonal Antibody [Clone ID: KT3]

Specifications

Product Data
Clone Name	KT3
Applications	FC
Recommended Dilution	Flow Cytometry: For optimal staining incubations should be performed at room temperature; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Reactivities	Mouse
Host	Rat
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
Specificity	This antibody recognises the CD3 antigen which is expressed by mature T cells.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: APC State: Lyophilized purified IgG Label: Allophycocyanin
Reconstitution Method	Restore with 1.0 ml distilled water
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	APC
Storage	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 12501 Mouse UniProt ID P22646
Background	T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms	T3/Leu-4
Reference Data