K2034-100 assays | HAT Activity Fluorometric Assay Kit Clinisciences

Background

Histone acetyltransferase (HAT) is an enzyme that acetylates conserved lysine on a histone protein by transferring an acetyl group from acetyl CoA. Histone acetylation can increase gene expression. HAT plays important roles in gene transcription, cell proliferation and differentiation.

The HAT Activity Fluorometric Assay Kit provides a sensitive and sensitive way for detection of HAT activity in a variety of samples based on fluorometric method that eliminates radioactivity in traditional assays. The assay utilizes H3 histone peptide and Acetyl CoA as substrates. The active HAT transfers acetyl groups from Acetyl-CoA to the H3 histone peptide, thereby producing two products - acetylated H3 histone peptide and CoA-SH. The CoA-SH reacts with the developer to yield a fluorophore product that can be easily detected using a fluorometer or a fluorescence plate reader at Ex/Em = 535/587 nm. The kit can determine HAT activity as low as 6 mU in a variety of samples.

Features & Properties

Features	Simple, rapid and convenient. The assay can detect HAT Activity as low as 6 mU in a variety of samples.
Shipping	Dry ice
Storage Conditions	Store at -80°C.

Quality Control

DataSheet

Related Biological Data

Related Biological Data

Storage

Store at -80°C.

Overview

Kit components

HAT Assay Buffer Acetyl CoA (Lyophilized) H3 Peptide (Lyophilized) Substrate Mix (Lyophilized) Developer PicoProbe™ CoA Standard (Lyophilized) Positive Control (HeLa Nuclear Extract)

HAT Activity Fluorometric Assay Kit

Cat# K2034-100 assays

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Brand : ApexBio Technology

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Background

Features & Properties

Quality Control

Related Biological Data

Related Biological Data

Related Biological Data

Storage

Overview