Phosphate, PBlue, BioAssay™ Kit
Cat# 299029-500T
Size : 500Tests
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
Shipping Temp
RTStorage Temp
4°CIntended Use:
Phosphatase Assays: liberation of phosphate from peptide, protein or small molecule substrate.
Lipase Assays: liberation of phosphate from phospholipids
Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
Quantitation of Phosphate: in phospholipids, proteins and DNAs, etc.
Drug Discovery: high-throughput screen for phosphatase inhibitors.
Lipase Assays: liberation of phosphate from phospholipids
Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
Quantitation of Phosphate: in phospholipids, proteins and DNAs, etc.
Drug Discovery: high-throughput screen for phosphatase inhibitors.
Sensitivity:
Detection of as little of 20pmoles of phosphate
Range:
0.4-50uM
Test Principle:
The PBlue Phosphate BioAssay™ Kit is based on a proprietary formulation of the malachite green dye. The PBlue reagent forms a blue colored complex with free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660nm) or on a plate reader. The non-radioactive colorimetric assay kits have been
optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high throughput screening of enzyme inhibitors.
optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high throughput screening of enzyme inhibitors.
Kit Components:
Reagent, 1x50ml
Standard, 1mM phosphate, 1x1ml
Standard, 1mM phosphate, 1x1ml
Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.