Protocadherin Gamma (pan) antibody
Cat# orb150750-100ug
Size : 100ug
Brand : Biorbyt
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Protocadherin Gamma (pan) antibody
Catalog Number: orb150750
| Catalog Number | orb150750 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to Protocadherin Gamma (ATTO-488). The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | S159-5 |
| Tested applications | ICC, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids). |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), ICC/IF (1:100) |
| Conjugation | ATTO-488 |
| MW | 100kDa |
| Target | Protocadherin Gamma |
| Entrez | 93709 |
| UniProt ID | Q91XZ0 |
| NCBI | NP_291062.1 |
| Storage | Conjugated antibodies should be stored at 4°C |
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated |
| Alternative names | PCDH gamma A antibody, PCDH-gamma-A antibody, PCDH |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-454 was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |