ASM10313-A565-100 | REEP2 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-REEP2 Monoclonal Antibody, Clone S326D-2 (ASM10313). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP2 Monoclonal Antibody (ASM10313) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) REEP2 Antibody (D) Composite.
Western Blot analysis of COS-REEP2-His showing detection of ~ 30 kDa REEP2 protein using Mouse Anti-REEP2 Monoclonal Antibody, Clone S326D-2 (ASM10313). Lane 1: MW Ladder. Lane 2: COS-REEP1-His. Lane 3: COS-REEP2-His. Lane 4: COS-REEP6-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP2 Monoclonal Antibody (ASM10313) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 30 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF
Primary Accession	Q8VCD6
Other Accession	NP_001191843.1
Host	Mouse
Isotype	IgG2A
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Mouse REEP2 Monoclonal IgG2A
Target/Specificity	Detects ~30kDa.
Other Names	Receptor expression-enhancing protein 2 Antibody, C2orf19 Antibody, SGC32445 Antibody, LOC682105 Antibody
Clone Names	S326D-2
Immunogen	Fusion protein amino acids 111-254 (cytoplasmic C-terminus) of mouse REEP2
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:100 dilution of SMC-481 was sufficient for detection of REEP2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Mitochondrion \| Mitochondrion Membrane \| Endoplasmic Reticulum \| Endoplasmic Reticulum Membrane

REEP2 Antibody