ASM10047-A565-100 | SAP102 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 (ASM10047). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody (ASM10047) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SAP102 Antibody (D) Composite.
Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 (ASM10047). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody (ASM10047) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, IP, ICC/IF, AM
Primary Accession	Q62936
Other Accession	NP_113827.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Rat SAP102 Monoclonal IgG1
Target/Specificity	Detects ~105kDa. No cross-reactivity against PSD95, SAP97 or Chapsyn-110.
Other Names	DLG3 Antibody, MRX90 Antibody, NEDLG Antibody, XLMR Antibody, Disks large homolog 3 Antibody, PSD-95/SAP90-related protein 1 Antibody, Synapse-associated protein 102 Antibody, SAP-102 Antibody
Clone Names	S19-2
Immunogen	Fusion protein amino acids 1-120 of rat SAP102
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml was sufficient for detection of Sap102 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Cellular Localization	Basolateral Cell Membrane \| Cytoplasm \| Cell Junction \| Synapse \| Postsynaptic Cell Membrane \| Postsynaptic Density \| Growth Cone

SAP102 Antibody