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> SHANK (pan) Antibody

SHANK (pan) Antibody

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Cat# ASM10203-A565-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - SHANK (pan) Antibody ASM10203
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49 (ASM10203). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody (ASM10203) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SHANK (pan) Antibody (D) Composite.
  • WB - SHANK (pan) Antibody ASM10203
    Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49 (ASM10203). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody (ASM10203) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, ICC/IF, AM
Primary Accession Q9QX74
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat SHANK (pan) Monoclonal IgG1
Target/Specificity Detects ~160kDa. Recognizes Shank1, 2 and 3.
Other Names Cortactin binding protein 1 Antibody, Cortactin SH3 domain-binding protein Antibody, Cortactin-binding protein 1 Antibody, CortBP1 Antibody, CTTNBP1 Antibody, GKAP/SAPAP interacting protein Antibody, GKAP/SAPAP-interacting protein Antibody, KIAA1022 Antibody, Proline rich synapse associated protein 1 Antibody, Proline-rich synapse-associated protein 1 Antibody, ProSAP1 Antibody, SH3 and multiple ankyrin repeat domains protein 2 Antibody, SHAN2_RAT Antibody, SHANK Antibody, Shank2 Antibody, SPANK-3 Antibody
Clone Names S23b-49
Immunogen Fusion protein amino acids 84-309 of rat Shank2
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-327 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cell Junction | Synapse | Postsynaptic Cell Membrane | Postsynaptic Density | Cell Projection | Growth Cone