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> Ubiquitin Antibody

Ubiquitin Antibody

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Cat# ASM10384-A565-200

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - Ubiquitin Antibody ASM10384
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - Ubiquitin Antibody ASM10384
    Western blot analysis of Human HEK93 lysates showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384) at 1:1000.
  • IHC - Ubiquitin Antibody ASM10384
    Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
  • IHC - Ubiquitin Antibody ASM10384
    Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.
  • ICC/IF - Ubiquitin Antibody ASM10384
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (ASM10384) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, ICC/IF
Primary Accession P0CG53
Other Accession NP_776558.1
Host Rabbit
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Bovine, Yeast, Xenopus, Dog, Fish, Sheep, Guinea Pig, Drosophila
Clonality Polyclonal
Description Rabbit Anti-Bovine Ubiquitin Polyclonal
Target/Specificity Detects ~10kDa. It also recognizes ubiquinated proteins.
Other Names Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
Immunogen Native bovine Ubiquitin, conjugated to KLH
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-119 was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Cytoplasm | Nucleus