AHA1 Antibody

Cat# ASM10146-A655-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

  • ICC/IF - AHA1 Antibody ASM10146
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AHA1 Monoclonal Antibody, Clone 4H9-D7 (ASM10146). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AHA1 Monoclonal Antibody (ASM10146) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AHA1 Antibody (D) Composite.
  • WB - AHA1 Antibody ASM10146
    Western Blot analysis of Human HeLa cell lysates showing detection of Aha1 protein using Mouse Anti-Aha1 Monoclonal Antibody, Clone 4H9.D7 (ASM10146). Primary Antibody: Mouse Anti-Aha1 Monoclonal Antibody (ASM10146) at 1:1000. (1) HeLa cell lysate (2) recombinant Aha1 cell lysate.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, E
Primary Accession O95433
Other Accession NP_036243.1
Host Mouse
Isotype IgG1 Kappa
Reactivity Human, Mouse
Clonality Monoclonal
Description Mouse Anti-Human AHA1 Monoclonal IgG1 Kappa
Target/Specificity Detects ~38kDa, can run up to 45kDa on SDS Page. It sees human Aha1, Mouse Aha1 and Mouse Aha2.
Other Names Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody
Clone Names 4H9-D7
Immunogen Human Aha1, his tagged
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-198 was sufficient for detection of Aha1 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Endoplasmic Reticulum