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> Protocadherin Gamma A3 Antibody

Protocadherin Gamma A3 Antibody

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Cat# ASM10285-A655-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - Protocadherin Gamma A3 Antibody ASM10285
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (ASM10285). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (ASM10285) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma A3 Antibody (D) Composite.
  • WB - Protocadherin Gamma A3 Antibody ASM10285
    Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A3 protein using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (ASM10285). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (ASM10285) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q91XY5
Other Accession NP_291064.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse
Clonality Monoclonal
Description Mouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1
Target/Specificity Detects ~100kDa. No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins.
Other Names PCDHGA3 Antibody, PCDG3 Antibody, PCDH gamma A3 Antibody, Protocadherin gamma subfamily A 3 Antibody, Protocadherin gamma-A3 Antibody, Gamma Protocadherin-A3 Antibody, Gamma-Protocadherin-A3 Antibody
Clone Names S144-17
Immunogen Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-A3. Human: 80% identity (68/84 amino acids identical) >75% identity with other Gamma-protocadherin-A protein.
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-451 was sufficient for detection of Protocadherin Gamma A3 in 20 µg of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma A3) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane