GRK (pan) Antibody

Cat# ASM10283-A655-100

Size : Onrequest

Brand : Abcepta

Contact local distributor :


Phone : +1 850 650 7790

  • ICC/IF - GRK (pan) Antibody ASM10283
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20 (ASM10283). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody (ASM10283) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GRK (pan) Antibody (D) Composite.
  • WB - GRK (pan) Antibody ASM10283
    Western Blot analysis of Monkey COS cells transfected with untagged GRK6 showing detection of ~65 kDa GRK (pan) protein using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20 (ASM10283). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with untagged GRK6. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody (ASM10283) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~65 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q8VEB1
Other Accession NP_061357.3
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Mouse GRK (pan) Monoclonal IgG1
Target/Specificity Detects 70kDa. Cross-reacts with GRK6.
Other Names G protein-coupled receptor kinase Antibody, Pan-GRK Antibody, GRK-pan Antibody, GRK Antibody
Clone Names S145-20
Immunogen Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5. 91% identical to human, and 99% identical to rat. >55% identity with GRK6 and GRK4. >40% identity with GRK7 and RK.
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-449 was sufficient for detection of GRK (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus | Cell Membrane