RayBio® C-Series Human NF-kB Array 2 Kit. Detects 34 proteins in human samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Human NF-κB Array C2
Human NF-κB Array C2
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 34 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Post-Translational Modifications, Phosphorylation, NFkB Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| ASC (PYCARD)
| BCL-10
| CD40 (TNFRSF5)
| cIAP-1 (BIRC2/hIAP-2)
| cIAP-2
|
| c-Rel
| FADD
| Fas (TNFRSF6/Apo-1)
| IkB-alpha
| IKB-b (NFKBIB)
|
| IkB-epsilon (NF-kappa-BIE)
| IKK-B
| IKK-E (IKBKE)
| IKK-gamma (NEMO/FIP-3)
| IL-1 Ra (IL-1 F3)
|
| IL-1 R1
| IL-17 RA
| IL-1 R5 (IL-18 R alpha)
| IRAK1
| IRF5
|
| JNK2 (MAPK9 isoform alpha-1)
| Lymphotoxin beta R (TNF RIII)
| MyD88
| NFKB1
| nfkb2
|
| NGFR (TNFRSF16)
| p53
| socs6
| TLR2
| |
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
| |
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human NF-kB Array C2 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit-IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or plastic Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.