Reagents and instruments for immunology, cell biology and molecular biology.
 
> Monolaurin [142-18-7]

Monolaurin [142-18-7]

Print Print
Please log in
Minimum order 2

Cat# HY-121620-1mL

Size : 10mM/1mL

Brand : MedChemExpress


Description

Monolaurin (1-Monolaurin) is an orally active antibiotic with antiviral and antibacterial properties[1][2][3].

Cellular Effect
Cell Line Type Value Description References
A498 ED50
3.77 μg/mL
Compound: 1
Cytotoxicity against human A498 cells after 7 days by MTT assay
Cytotoxicity against human A498 cells after 7 days by MTT assay
[PMID: 9134750]
MIA PaCa-2 ED50
2.33 μg/mL
Compound: 1
Cytotoxicity against human MIAPaCa2 cells after 7 days by MTT assay
Cytotoxicity against human MIAPaCa2 cells after 7 days by MTT assay
[PMID: 9134750]
PC-3 ED50
23.28 μg/mL
Compound: 1
Cytotoxicity against human PC3 cells after 7 days by MTT assay
Cytotoxicity against human PC3 cells after 7 days by MTT assay
[PMID: 9134750]
In Vitro

Monolaurin (1.25 μg/mL, 24 hours) exhibits significant inhibitory effects on Staphylococcus aureus[3].
Monolaurin (2.5 μg/mL, over 48 hours) exhibits significant bactericidal effects on Staphylococcus aureus[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Monolaurin Related Antibodies
In Vivo

Monolaurin (5 mg per mouse, orally administered, once daily for 30 days) can effectively inhibit Staphylococcus aureus infection in C3H/hej mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C3H/hej mice (weighing 15-18 g​, intraperitoneally injected with 25 × 106 CFU Staphylococcus aureus suspension to induce infection)
Dosage: 5 mg per mouse
Administration: Oral gavage (p.o.), once daily for 30 days, dosing started 1 week before infection
Result: Provided 50%-70% protection against infections caused by Staphylococcus aureus.
Clinical Trial
Molecular Weight

274.40

Formula

C15H30O4
CAS No.
Appearance

<52°C Solid,>52°C Liquid

Color

White to off-white

SMILES

CCCCCCCCCCCC(OCC(CO)O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (45.55 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6443 mL 18.2216 mL 36.4431 mL
5 mM 0.7289 mL 3.6443 mL 7.2886 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (4.56 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.25 mg/mL (4.56 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Purity & Documentation
References